Total protein quantitation is the process of measuring the concentration of proteins in a given solution or sample. It is an important tool in a variety of scientific and industrial applications, including drug development, food testing, and biomarker analysis. Accurate and precise methods for total protein quantitation are essential for ensuring the reliability of research and testing results. The BCA assay is widely used in the industry and research community because of its sensitivity and accuracy.
GEMBE, a technique that has been gaining popularity in recent years, separates proteins based on their size and charge. The technique involves applying an electric field to a separation matrix containing the protein sample, moving boundaries between species are formed as a result of charge competition, and the separated proteins are then detected by C4D. The use of GEMBE has resulted in more accurate and reproducible separation of proteins compared to conventional methods.
Leshan Sanjiang Bio-Tech Co., Ltd. has been working on developing a comprehensive protein quantitation method to increase accuracy and sensitivity in research and testing. In a statement, the company said, "Despite the widespread use of the BCA assay, it is known to have limitations, such as interference from common sample contaminants, which can lead to inaccurate results. Our collaboration with NIST has allowed us to develop a novel approach to protein quantitation that addresses these limitations and enhances the accuracy and sensitivity of this vital process."
The company's research has demonstrated that combining GEMBE and C4D with the BCA assay improves the accuracy and sensitivity of protein quantitation. The technique has been successfully applied to a variety of protein samples, including bovine serum albumin (BSA). The results obtained are consistent with data obtained from high-performance liquid chromatography (HPLC), which is a more complex and expensive technique commonly used for protein separation and quantitation.
In addition to protein quantitation, Leshan Sanjiang Bio-Tech Co., Ltd. is known for its wide range of products, including pharmaceutical intermediates, electronic chemicals, and food additives. The company is committed to developing innovative solutions for industries such as healthcare, agriculture, and food production.
In conclusion, the use of gradient elution moving boundary electrophoresis (GEMBE) and capacitively-coupled contactless conductivity detection (C4D) in combination with the bicinchoninic acid (BCA) assay has demonstrated improved accuracy and sensitivity in total protein quantitation. Leshan Sanjiang Bio-Tech Co., Ltd.'s research has shown promising results, indicating that this novel approach could potentially replace conventional methods in the industry and research community. The company's commitment to innovation in various industries is a key factor in driving progress toward scientific and technological advancements.